Western blot analysis of mouse ZBTB8OS/ARCH was performed by loading whole-cell lysates of mouse embryonic fibroblasts (each of the three lanes was equally loaded with 10000-15000 cell equivalents) in reducing sample buffer onto a 20-well NuPAGE™ 4-12% Bis-Tris Midi Protein Gels (Product # WG1402A). Proteins were transferred to PVDF membrane with tank transfer. Membrane was blocked in 5% non-fat milk/TBST. Mouse ZBTB8OS/ARCH was detected at approximately 26 kDa using a ZBTB8OS/ARCH monoclonal antibody (Product # MA5-24536) at a dilution of 1:2000 in 5% non-fat milk/TBST overnight at 4°C on a rocking platform, followed by a 2-hour room-temperature incubation of a goat anti-mouse IgG (H+L)-HRP conjugate at a dilution of 1:5000. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32209). Data courtesy of Antibody Data Exchange Program.